Tomomi NEMOTO Laboratory of the Research Institute for Electronic Science, Hokkaido University, initiated a new study in September 2009. We are developing a sophisticated two-photon microscope that can perform fluorescence imaging of live organisms more deeply than any other microscope in the world. To date, the microscope has achieved an imaging depth of nearly 1.6 mm, enabling in vivo observation of hippocampal dentate gyrus neurons. We are also pursuing development of “super-resolution imaging” and “optical nano-imaging” using novel laser beams called “vector beams,” in order to observe minute structures and interactions between biomolecules that were previously not observable due to the classical limit imposed by the diffraction of light. In addition to this imaging method, which uses non-linear optical processes induced by ultra–short-pulse laser beams, we have used genetic engineering, electrophysiology, and molecules with optical functions to develop new methods for analyzing biological functions. These novel methods enable simultaneous “optical in vivo observation” and “optical in vivo operation” on living individuals and tissues.
By applying these methodologies, we aim to discover the physiological functions of the brain, nervous system, and endocrine and exocrine systems, as well as the molecular mechanisms underlying conditions such as bone diseases, cancers, and diabetes. We seek to establish and enhance novel interdisciplinary research areas, called “Optical brain science” and “Optical cellular biology”, which will lead to clinical applications connecting optics and life sciences.
The Nemoto Lab is now accepting graduate students and postdoctoral fellows who are interested in these projects, as well as proposals for collaborative studies. Please contact us if you are interested in our research.

Contact (Map
N20W10, Kita-ku, Sapporo, Hokkaido, 001-0020, JAPAN
Tomomi Nemoto, Laboratory of Molecular and Cellular Biophysics, Research Institute for Electronic Science, Hokkaido University.

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in vivo imaging of all layers of neocortex and neurons in hippocampus CA1& dentate gyrus using novel two-photon microscopy YouTube-icon-full_color_34
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R. Kawakami, et al., Biomedical Optics Express (2015)

Recent events

  • An original paper on a collaborative work with Prof. Okamura, Tokai Univ. has accepted in Advanced Materials. Aug. 11. This paper was introduced the "NIKKEI SANGYO SHINBUN" 9/6
  • An original paper on a collaborative work with Prof. Yokoyama, Tohoku Univ. has accepted in Appl. Phys. Express. Sep. 6
  • A review article in Japanese has been published in "KENBIKYO". Sep. 1
  • An original paper on a collaborative work with Prof. Uehara, Hokkaido Univ. has accepted in FEBS Lett. Sep. 1
  • Prof. Nemoto was invited to deliver an oral presentation at 24th Congress of the International Commission for Optics (ICO24). Aug. 23.
  • Prof. Nemoto was invited to deliver an oral presentation at CLEO PR, OECC & PGC 2017. Aug. 2.
  • Nikon imaging center mini symposium on fluorescence was held. Jul. 27.
  • Prof. Nemoto was invited to deliver an oral presentation at the medical school of Kyoto Univ. Jul. 20.
  • Dr. Otomo was invited to deliver an oral presentation at International Symposium on Imaging Frontier 2017 (ISIF 2017). Jul. 20.
  • An image analysis training course by "ABiS" was hold on Jul. 6-7.
  • The original paper by Ms. Aoyagi in PLoS ONE (2015) was ranked up to the most cited 25% papers in PLoS ONE Jul. 3
  • Dr. Kawakami was promoted to associate professor in the medical school of Ehime University. Jul. 1
  • Prof. Kenji Matsuno, Osaka Univ,, kindly gave us an oral presentation at Idea Seminar. Jun. 28
  • Professor Murata gave his poster presentation and selected as a special oral presenter at the 69th annual meeting of the Japan society for Cell Biology. Jun. 13-15
  • Professor Nemoto and Prof Michiyuki Matsuda organized a workshop in the 69th annual meeting of the Japan society for Cell Biology. Professor Nemoto was invited to deliver an oral presentation there. Jun.14
  • Dr. Hirokaza Ishii and Dr. Lin Qiao joined our group Jun. 1
  • A training course of light microcopy was hold by Hokudai Nikon Imaging Center assisted by KAKENHI programs "Resonate Bio", and "ABiS". May15
  • We organized the annual meeting of KAKENHI program "Resonance Bio". May 13, 14
  • Prof. Nemoto was invited to deliver an oral presentation at the 15th annual meeting of society of nano science and technology. May 10
  • Prof. Nemoto was invited to deliver an oral presentation at ALPS2017. Apr. 14-21
  • Dr. Otomo gave his poster presentation at FOM2017. Apr. 9-12
  • Mr. Yamaguchi has moved to the doctor program, and Mr. Ataka, Ms. Goto and Mr. Takahashi have moved to the master program. Dr. Tsutsumi has promoted to project assistant professor. Ms. Emi Sato joined our research group. Apr. 1
  • Ms. Ipponjima and Ms. Tanabe graduated the doctoral course of the graduate school. Mr. Yamaguchi finished the master course of the graduate school. Mr. Ataka, Ms. Goto and Mr. Takahashi graduated the undergraduate school in Hokkaido University. Congratulation!

Research results and events to be published
• Three reveiew papers by Dr. Tsutumi, Dr. Otomo, or Dr. Kawakami are in press.
• An English technical book in which Dr. Kawakami wrote a section is in press (Springer Nature)
• Annual meeting of NIC -G3 dynamic alliance symposium Nov. 28
• Neurophotonics workshop & Biospectroscopy workshop of the Spectroscopical Society of Japan Oct. 27, 28
• Dr. Otomo Invited Biophysical society of Japan Sep. 19-21
• Mr. Kitamura poster the 26th annual meeting of the bioimaging society Sep. 16
Recent Events Archives

moyashi
ABiSロゴFullのコピーPasted Graphic 2Brain-Mind
Two-photon microscopy, Multi-photon microscopy, in vivo imaging