RECENT ADVANCES IN SUPER-RESOLUTION FLUORESCENCE MICROSCOPY BY FINCH HOLOGRAPHY

• Professor: Department of Biomedical Engineering, Johns Hopkins University, 9605 Medical Center Drive, Rockville, Maryland 20850 USA
• Director: Microscopy Center, Johns Hopkins University Montgomery County Campus, Rockville, Maryland 20850 USA

In this lecture I will chronicle progress over the last 6 years since the invention of Fresnel Incoherent Correlation Holography by Joseph Rosen and Gary Brooker. In its current state of development, picture perfect 3D images with resolution beyond the Rayleigh limit can be reconstructed from FINCH holograms acquired by fluorescence microscopy. A number of optical, electro-optical and computational advances by ours and other laboratories have made this possible. Those advances and new approaches from our group will be discussed and have enabled us to improve image resolution to about two times the Rayleigh limit in fluorescence microscopy. The simplicity of FINCH and the ability to use any fluorescent dye and microscope objective with varying magnification (10X-100X) and NA (0.4-1.4) make FINCH an attractive method for 3D super-resolution microscopy.